FastQCFastQC Report
Wed 25 May 2016
SRR2074826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673962
Sequences flagged as poor quality0
Sequence length60
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC9520.1412542546909173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA2500.044.27884342
CGTCGTG2750.042.2170839
TCTCGTA1600.042.186438
GCGTCGT2700.041.99890538
GTAGCGT2750.041.23528735
TAGCGTC2750.040.25349436
AGCGTCG2850.038.8410937
GTCGTGG3050.038.0645840
ATTGGCG1000.037.7990121
AGTAGCG3100.037.45063434
ATTTACG950.036.94640454
CTCGTAT1850.036.48553539
CGTATGC1950.035.99905841
CCGTCTT1450.035.37838747
TATGCCG1800.034.499143
GCGTGAA1350.033.9991125
CGTTTAG402.1173722E-433.7491210
TCGTGGT3300.033.5445841
TAGCGGA1050.033.427734
TAAGCAT3400.033.3520715
Produced by FastQC (version 0.11.5)