FastQCFastQC Report
Wed 25 May 2016
SRR2074825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993739
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.034.1871951
TCGACGG2800.033.74943529
ACGGCAT2650.032.60322632
CTCGACG2900.032.5856628
TATGCCG1350.029.99949643
GACGGCA3350.029.01443731
CGACGGC3400.028.58775530
ACTCGAC2950.028.37240427
TCTCGTA1052.8558134E-1028.28523838
TAGCGGA1204.5474735E-1126.99954834
ACGGCTT1104.8930815E-1026.99954643
ATTGGCG1158.203642E-1025.82565521
CGGCATC2950.025.62668833
CTCGTAT1201.3442332E-924.74958439
ACCGCGT550.001364986424.5450428
TCGTATG1101.4473699E-824.54504240
GTATCAA35000.023.8379421
CGTATGC1252.1573214E-923.75960241
TTGGCGT1403.1832315E-1023.1424722
AGCGGAT1403.1832315E-1023.1424735
Produced by FastQC (version 0.11.5)