FastQCFastQC Report
Wed 25 May 2016
SRR2074824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799600
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7000.036.25651643
CGTATGC7050.035.9993841
TCGTATG7050.035.9993840
CTCGTAT7100.033.4642139
TCTCGTA7250.032.02703538
TGCCGTC8850.028.9829345
GCCGTCT8150.028.49067946
ATGCCGT9000.028.19988444
GGTATCA68650.027.061021
ACGAGAC10300.026.47561822
ATCTCGT8500.026.3642537
CCGTCTT8950.026.245747
ATTGGCG3300.023.72717321
CGTCTTC10050.023.64169148
GACTAAG11750.023.43789126
CGAGACT12100.022.98307423
GTATGCC10850.022.89361442
CCACGAG12250.022.48153920
CCCACGA12100.022.09081619
CACGAGA12300.021.95112821
Produced by FastQC (version 0.11.5)