FastQCFastQC Report
Wed 25 May 2016
SRR2074823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4063439
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT8150.032.1342838
GTAGCGT7950.031.92384535
CGTCGTG8350.031.04125239
TAGCGTC8550.029.99935736
GGTATCA68700.029.634741
AGTAGCG9050.028.93860634
AGCGTCG8900.028.81960737
CGTGGTA9250.027.72913642
TATGCCG4400.024.5449343
GTATCAA120400.023.5028931
TCGTGGT10850.023.1423641
TCTCGTA5600.020.73169738
GGGTAGT12000.020.70006624
GGTATTC8850.020.13540845
GGTAGTC12900.019.67399625
GTCGTGG13750.019.63594240
CGAGACT9400.019.53197723
CTCGTAT6150.019.3166639
GTGTAAG14550.018.3714212
TCGTATG7550.018.2380240
Produced by FastQC (version 0.11.5)