FastQCFastQC Report
Wed 25 May 2016
SRR2074822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923349
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4800.037.12507243
TCGTATG4750.036.9474440
CGTATGC5500.031.4182441
GGTATCA50600.030.8953151
CTCGTAT5550.030.16221839
TCTCGTA6150.028.5366438
CGCTATG7050.028.34047930
GCCGTCT6100.026.9995946
CTCGCTA7800.026.65389628
TCGCTAT7800.025.96158829
ATGCCGT6700.025.79109444
ATCTCGT6850.024.8317437
GACTCGC8200.024.6951726
ACTCGCT7850.024.4204327
ATTGGCG3750.023.7596421
ATTTACG3600.023.24964754
CCGTCTT6950.022.92051347
TAGCGGA4150.022.77073934
TGCCGTC7550.022.52945945
ACGAGAC8650.022.16189422
Produced by FastQC (version 0.11.5)