FastQCFastQC Report
Wed 25 May 2016
SRR2074820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3800508
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.030.6744671
TCGTATG5350.027.75656340
TATGCCG5150.027.26169843
CGTATGC6000.024.2996141
TACCGCT6850.024.04340729
CTCGTAT6100.023.90125739
ACTACCG7150.023.4122127
CTACCGC7350.023.14248528
GTATCAA100850.022.6761881
TCTCGTA6100.022.57340838
ATGCCGT6450.019.67410344
ATCTCGT6550.018.96152937
ACCGCTG9250.018.38889330
CGCTGAT7350.017.9997132
CCGCTGA8900.017.89858831
ATCAACG126100.017.66453
TCAACGC127050.017.5749194
GCCGTCT6800.017.47030646
ACGAGAC8550.017.36859922
TGCCGTC7000.017.35686345
Produced by FastQC (version 0.11.5)