FastQCFastQC Report
Wed 25 May 2016
SRR2074819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927322
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.1191809152803735No Hit
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC19400.10065780393727669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA4200.042.4277442
GTAGCGT4300.041.44104435
GCGTCGT4650.038.90246238
AGCGTCG4700.038.48860537
TAGCGTC4600.038.15142436
CGTCGTG4750.037.5150539
ATTTACG1500.035.9992954
TATGCCG3550.034.98522643
AGTAGCG5500.033.380334
GGGTAGT5850.030.9232724
TCGTGGT6300.028.7137241
TAGCGGA2300.028.17262634
GTCGTGG6550.028.02998440
CGTATGC4000.027.67445641
TCGTATG3550.027.37974440
ATGCCGT4550.027.29687344
GGTATTC4850.027.27852245
ATCGCGT607.1618306E-526.9994737
ATTGGCG2450.026.44845821
GTGTAAG7000.026.22805612
Produced by FastQC (version 0.11.5)