FastQCFastQC Report
Wed 25 May 2016
SRR2074818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025660
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74450.027.4186151
TATGCCG3050.023.90121743
TCGTATG4150.021.46949840
GTATCAA115750.020.2249091
CGTATGC4750.017.0523341
TCAACGC139750.016.6163274
ATCAACG140850.016.5440713
TATCAAC141450.016.5120722
CAACGCA144050.016.4583345
AACGCAG149850.015.9474476
TCTCGTA5750.014.55626438
CTCGTAT5650.014.33602939
ACGAGAC7150.013.9717822
GTACATG169600.013.803261
GAGTACT89750.013.74828712
TACATGG169750.013.5524642
ACGCAGA177200.013.4245787
CGCAGAG181450.013.3030988
ATCTCGT6300.013.2854837
TAGACCG1855.525171E-513.1363715
Produced by FastQC (version 0.11.5)