FastQCFastQC Report
Wed 25 May 2016
SRR2074817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138494
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC22630.10582213464241659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4800.035.99962639
CGTGGTA4850.035.62849442
GCGTCGT4900.035.2649438
TAGCGTC4750.034.67332536
TAGCGGA2700.033.9996534
TATGCCG4200.033.42822343
AGCGTCG5250.032.39966237
GTAGCGT4950.031.63603435
TCGTGGT5500.031.41785241
ATTTACG2250.031.20040554
TCGTATG4050.029.99969140
ATTGGCG2850.029.36811421
AGTAGCG5650.028.67226834
GGTATCA48400.028.1725121
GGGTAGT6500.026.58434124
GGTATTC5600.026.51758445
TATAACG1750.026.2295252
CTCGTAT5050.026.19774839
GGTAGCG3300.026.18154532
CGTATGC5400.025.49973541
Produced by FastQC (version 0.11.5)