FastQCFastQC Report
Wed 25 May 2016
SRR2074816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754184
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC34920.12678891461136946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT7550.042.91282335
CGTCGTG7050.042.126639
TAGCGTC7650.041.64600836
AGCGTCG8050.039.5766437
AGTAGCG8550.039.15690634
CGTGGTA7750.039.0183742
GCGTCGT7800.038.42210838
TCGTGGT8450.035.4665641
TATGCCG4150.034.4810643
GGGTAGT10200.032.2956524
GTCGTGG10050.031.43204140
GGTATTC8150.029.15263745
TCGTATG5150.028.83422340
CTCGTAT5050.028.33591839
TCTCGTA5050.028.33591838
GGTATCA54300.028.1434461
CTGGGTA12450.027.54347622
GGTGTAA12600.027.0007911
GTGTAAG12550.026.89321712
GAGTAGC12350.026.89000933
Produced by FastQC (version 0.11.5)