FastQCFastQC Report
Wed 25 May 2016
SRR2074815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593883
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6750.038.3994843
CTCGTAT7000.035.48523339
TCGTATG7500.035.27952240
GTAGCGT3550.034.9854435
TCTCGTA7250.034.26160438
CGTATGC7850.033.7065541
GCGTCGT3550.032.7037838
ATGCCGT8050.031.86292344
CGTCGTG3650.031.06807139
TAGCGTC3950.030.75907736
ACGTTCA8700.030.7237227
CCGTCTT7800.030.11497547
ATTGGCG2600.030.11497521
CGTGGTA3550.028.90101642
TGCCGTC8850.028.6775845
GCCGTCT8500.027.9525646
CGAGACG10350.027.65179823
GGTATCA56200.026.8084981
GTATGCC10200.026.73493242
AGCGTCG4500.026.39964137
Produced by FastQC (version 0.11.5)