FastQCFastQC Report
Wed 25 May 2016
SRR2074813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541872
Sequences flagged as poor quality0
Sequence length60
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG4150.029.92715529
GGTATCA51400.029.7333051
ACTCGAC4250.028.58770627
CTCGACG4450.027.90959528
TATGCCG1850.024.81034943
TCGCGTA702.0402209E-423.14242738
CGACGGC5750.023.00826830
ATCGCGT753.2539436E-421.59959837
ACGAGAC6700.021.35823222
CGAGACT7250.020.85519423
GTATCAA85600.020.7874641
CCACGAG7200.019.12502120
CGCGTAT857.566589E-419.05846839
CCCACGA7250.018.99312419
GACGGCA6150.018.87769731
CACGAGA7300.018.86303321
AGACTCG8000.018.56215525
ACGGCAT4950.018.54510932
GACTCGA6750.018.39965826
GCGGTAT1202.0471629E-518.0010811
Produced by FastQC (version 0.11.5)