FastQCFastQC Report
Wed 25 May 2016
SRR2074812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5357388
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7650.033.88163443
TCGTATG9300.031.6444940
ATCTCGT8950.029.2619537
CGTATGC9450.028.85652741
CTCGTAT10000.028.07940339
GGTATCA84850.026.666311
TCTCGTA10500.026.22801438
ATGCCGT9900.025.90926644
CCGTCTT10100.023.7922447
GCCGTCT10400.023.36554346
ACGAGAC13700.022.66396522
GTATCAA143300.022.1392251
GACTAAG15450.020.6209226
TGCCGTC12250.020.49809845
CCACGAG14100.020.29781720
CGTCTTC13550.018.93001748
CACGAGA15450.018.52405221
ATCAACG178350.018.3787633
TCAACGC177800.018.2533874
GTATGCC14800.018.24285542
Produced by FastQC (version 0.11.5)