FastQCFastQC Report
Wed 25 May 2016
SRR2074811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3805622
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5100.029.11723143
TCGTATG5950.027.68027540
CGTATGC6050.025.88392841
CTCGTAT6250.024.62364839
GGTATCA74850.024.5660691
TCTCGTA6300.023.99965738
ATCTCGT6800.022.23497437
ACGAGAC11150.020.8253622
GTATCAA119700.020.775231
CGAGACT11600.019.31894323
CCACGAG12450.019.30143420
ATGCCGT7850.019.2608744
TGCCGTC7800.018.3458945
CACGAGA13100.018.13761921
CGTATAG3650.017.7541031
CCCACGA12300.017.56118619
ATCAACG148550.017.231223
TCAACGC148750.017.1172944
GTCGTAC1455.5299806E-616.7592621
CAACGCA154250.016.7520185
Produced by FastQC (version 0.11.5)