FastQCFastQC Report
Wed 25 May 2016
SRR2074810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494690
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC39460.15817596575125567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT8450.042.1770935
CGTGGTA8700.040.65476642
GCGTCGT8600.040.4995938
AGCGTCG8900.040.3479137
TAGCGTC8850.039.6606236
TATGCCG5200.038.94191743
CGTCGTG9100.038.5710439
AGTAGCG9550.037.31934
TCGTGGT10050.035.4623341
GGTATTC8600.032.65083745
TCGCTAT7100.031.94334429
GTCGTGG11250.031.9196840
GTAAGCA11850.031.21487414
GTGTAAG11750.030.79179612
TCGTATG6850.030.74421940
TAGCGGA1850.030.64834234
TCTCGTA6850.030.35006138
CTCGCTA7750.030.30937628
ATGCCGT7100.030.04195444
TAAGCAT12300.029.85335715
Produced by FastQC (version 0.11.5)