FastQCFastQC Report
Wed 25 May 2016
SRR2074809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756531
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG7150.040.0271640
TATGCCG6550.038.33510643
CGTATGC7300.037.3554141
ATTGGCG5150.037.22322521
TCTCGTA8050.035.88747438
CTCGTAT7850.035.7699839
ATTTACG5450.035.17360354
TAGCGGA5700.034.5782534
ATCTCGT8150.034.1220137
AGCGGAT6200.031.7896835
GGTAGCG6550.030.91540732
TGCCGTC8000.030.03689445
ATGCCGT8850.028.98246844
GTTATAC11500.027.7037930
CGGATGA7500.026.63946337
TTGGCGT7300.026.63008322
ACGAGAC12100.026.33052822
CCGTCTT7650.025.41125347
CATCTCG11200.025.3119936
GTAGCGG8050.024.81937633
Produced by FastQC (version 0.11.5)