FastQCFastQC Report
Wed 25 May 2016
SRR2074808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189519
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC26410.12062009966572566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.10632472246187405No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23020.10513724703918989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC5200.040.49934836
CGTGGTA4800.039.9368642
AGCGTCG5600.037.12440537
CGTCGTG5250.037.02797339
GCGTCGT5400.036.99940538
GTAGCGT5850.035.53788835
ATTTACG3350.034.6561654
ATTGGCG3750.033.83945521
TATGCCG4900.033.61170643
TAGCGGA4200.032.1423434
TCGTATG5600.030.37451240
AGTAGCG7000.030.08523234
GGTAGCG4550.029.66985332
TACCGCT6450.029.30185529
ATGCCGT5900.028.83004644
TATCACG1500.028.8028262
GGGTAGT6850.028.77326224
CTACCGC7150.027.94360728
GCGTGAA4750.027.85218425
TCGTGGT7000.026.99956741
Produced by FastQC (version 0.11.5)