FastQCFastQC Report
Wed 25 May 2016
SRR2074807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463884
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC28770.1167668607775366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6150.041.2676137
GCGTCGT6350.039.54265238
TAGCGTC6450.039.34818636
GTAGCGT6800.038.11701635
CGTCGTG6650.037.35276839
CGTGGTA6450.036.836642
TCGCGTA852.3646862E-1134.94059838
TAGCGGA3950.034.1766534
AGTAGCG7550.033.97294634
TCGTGGT7900.032.1260541
ATTTACG3450.032.08642654
GTCGTGG8450.031.31309140
ATTGGCG4100.030.29341121
GGGTAGT8550.029.99950424
AGCGGAT4700.029.29738835
TATAACG1303.6379788E-1227.0022932
GGTATTC7150.026.4331345
GGTAGCG5050.025.66294332
TATGCCG2850.025.57852643
GGTAGTC10150.025.53652625
Produced by FastQC (version 0.11.5)