FastQCFastQC Report
Wed 25 May 2016
SRR2074806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058413
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC62130.20314457203785102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG12200.042.49081439
GTAGCGT12450.041.63758535
AGCGTCG13000.040.9144337
GCGTCGT12900.039.9758138
TAGCGTC13200.039.68089336
TATGCCG4650.038.90231743
AGTAGCG13400.038.68566534
CGTGGTA13250.038.10477442
GGTATTC12300.034.90219545
TCGTGGT15200.033.92683841
GTCGTGG15650.033.4688740
CTCGTAT6050.031.68521139
GGGTAGT16550.031.15939724
GGTAGTC16700.030.87951925
TAAGCAT17750.029.81436515
GAGTAGC17850.029.49511133
GTATTCC14450.029.33543646
CTGGGTA18150.029.15634522
GTAAGCA18200.029.07719614
CCGTCTT5950.029.04181347
Produced by FastQC (version 0.11.5)