FastQCFastQC Report
Wed 25 May 2016
SRR2074805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208887
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5100.030.70556341
TCGTATG5250.029.8282640
TATGCCG5800.029.79279343
ATTGGCG4900.027.55073521
ATTTACG5250.026.22829854
ATCTCGT6550.025.96919637
CTCGTAT6200.025.69328139
TCTCGTA6450.025.53461838
GGTAGCG5300.025.47143432
AGCGGAT6050.024.99147835
TAGCGGA6050.024.09892534
GGTATCA73200.023.3123231
ACGAGAC10200.023.02917322
TGCCGTC7200.021.74977345
ATGCCGT7850.021.66856644
GCGTGAA7300.020.71211225
CGGATGA7700.019.98680537
GTATCAA115700.019.9765261
CCGTCTT5650.019.59271647
GTAGCGG7200.019.49979833
Produced by FastQC (version 0.11.5)