FastQCFastQC Report
Wed 25 May 2016
SRR2074803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002929
Sequences flagged as poor quality0
Sequence length60
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31150.10373205626906262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.10283293411199532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA300.002107811235.99943542
CGTATGC7000.032.01378341
TATGCCG6700.031.83532143
TCGTATG7600.030.90740840
CTCGTAT7800.028.73031639
AATCTCG7700.028.4021536
GGTATCA58700.027.0469231
CCGTCTT7300.026.99957747
CGTTCAT9700.026.72122828
TCTCGTA8200.026.3410538
ACGTTCA9350.026.2776627
CGAGACG9950.024.96443223
ATGCCGT8950.023.83202744
ACGAGAC10500.023.65716622
GCCGTCT8850.023.49115846
ATTAATC9850.022.47680533
GAGACGT11100.022.37802724
TGCCGTC10000.021.5996645
GTATCAA94600.021.2068581
CACGAGA11850.020.96204421
Produced by FastQC (version 0.11.5)