FastQCFastQC Report
Wed 25 May 2016
SRR2074802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815230
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.10528447054059527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.10450300685911987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5050.035.2865343
ATTGGCG4650.034.25869421
TAGCGGA4900.033.61166834
TCGTATG6400.032.48381840
ATTTACG3950.031.442554
GCGTGAA5450.030.22020125
CGTATGC6500.029.9071841
CTCGTAT6800.029.77890239
GGTAGCG5600.029.41021232
AGCGGAT5650.029.14994235
GCGTCGT2900.028.86157638
TATCTCG7150.028.6988136
TCTCGTA7350.028.2852338
GACCCTA9350.027.43317226
ATGCCGT6650.026.39052644
TAGCGTC3150.025.71384436
ACGAGAC11000.025.28183622
GGTATCA63650.024.477361
TATAACG2650.024.454152
CGTCGTG3300.023.72686839
Produced by FastQC (version 0.11.5)