FastQCFastQC Report
Wed 25 May 2016
SRR2074801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878497
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG2050.032.92634229
GGTATCA33400.032.5799641
CTCGACG2200.031.90861928
CGACGGC2600.026.999630
ACTCGAC2900.023.27551727
GTATCAA55550.022.4082971
GACTCGA3100.020.90291826
CGAGACT3500.020.8277123
AGACTCG3750.020.15970225
GCGAAAT700.005532926419.28645513
TATCAAC69750.017.7301852
ATCAACG70900.017.4806863
TCAACGC70200.017.4242124
CAACGCA72750.017.4073185
GACGGCA4000.016.87430231
AACGCAG76000.016.805046
GCACCGA2651.2732926E-1116.3020811
CGCCGAC3350.016.11959512
TATGCCG1355.6258963E-515.99933843
ACCGACT2903.6379788E-1215.82819513
Produced by FastQC (version 0.11.5)