FastQCFastQC Report
Wed 25 May 2016
SRR2074800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193607
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC42740.13382986698112823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9350.041.870739
GCGTCGT9950.039.88853538
TAGCGTC9600.039.37409636
CGTGGTA10000.038.8791142
GTAGCGT10050.037.8797335
AGCGTCG11050.036.16206737
TATGCCG3300.035.1810143
AGTAGCG11150.034.86915634
TCGTGGT11350.033.54108441
GTCGTGG12450.032.31251540
GGTATTC10550.029.43106745
GGGTAGT13650.029.07625824
GGTATCA62500.027.1311021
CTCGTAT4500.026.9993839
GTGTAAG14250.026.9059112
GGTAGTC15200.026.64412525
CTGGGTA15500.025.95505722
TCTCGTA4550.025.51589838
GTATTCC12300.025.2437246
TGGGTAG16250.024.42405723
Produced by FastQC (version 0.11.5)