FastQCFastQC Report
Wed 25 May 2016
SRR2074799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335790
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG4000.040.50078621
TAGCGGA4650.036.5797934
AGCGGAT4900.034.16246835
TTGGCGT4700.033.8935522
ATTTACG4800.032.0617554
GGTAGCG5350.030.78432732
TCGTATG2850.030.3150840
GCGGATG5950.028.58756436
GTAGCGG5950.028.13379533
CGTATGC2500.028.07934241
TATGCCG2750.027.49026543
GAGGCGC6150.027.21945613
CGGATGA6650.025.98435437
TCTCGTA3250.024.92249538
CTCGTAT3450.024.26030339
TCTGTCG4950.022.3635928
GGTATCA63850.021.6098021
GGCGCCA7500.021.60088215
TATAACG2100.020.5727082
ATCTCGT3950.020.5058537
Produced by FastQC (version 0.11.5)