FastQCFastQC Report
Wed 25 May 2016
SRR2074798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3441264
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.029.3388921
GTATCAA99700.023.1561451
TATACCG1603.7083737E-818.564125
TATCAAC124250.018.515632
ATCAACG125100.018.3898263
TCAACGC125450.018.2742124
CAACGCA129350.018.2659915
TCGCTAT4000.018.22447229
AACGCAG137200.017.3389746
TATGCCG3000.017.09950443
CTCGCTA4850.015.58717528
ACGCAGA155600.015.2710327
CGCAGAG161500.014.9635018
CGTTCGG1307.656287E-414.53846110
GTACATG127650.014.4898881
TACATGG128850.014.2501612
AGAGTAC166850.014.19178911
CAGAGTA169200.013.9946810
GAGTACT95050.013.833570512
GCAGAGT171700.013.8223659
Produced by FastQC (version 0.11.5)