FastQCFastQC Report
Wed 25 May 2016
SRR2074797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585127
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG7900.038.6196837
CGTCGTG7800.038.42250439
GTAGCGT8050.037.56465535
TAGCGTC8200.036.877536
GCGTCGT8250.036.3267338
CGTGGTA8950.034.3905542
AGTAGCG9100.033.8236734
GGTATCA63850.031.1682421
TATGCCG4750.030.69427543
GTCGTGG10800.028.99956540
GGTATTC9900.025.90870345
TCGTGGT11700.025.61541
CTCGTAT5950.024.50383639
CGAGACT7800.024.23040823
TCTCGTA6750.023.99963838
GGGTAGT13200.023.9314624
GTATCAA110800.023.0058351
CTGGGTA14500.022.716922
GGTAGTC14200.022.62642325
GCCGTCT5500.022.58147846
Produced by FastQC (version 0.11.5)