FastQCFastQC Report
Wed 25 May 2016
SRR2074796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927708
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG2900.039.103554
CGTGGTA3950.036.2269642
ATTGGCG2900.035.37844521
TATGCCG3100.032.22501843
AGCGTCG4450.032.15651737
GCGTCGT4400.031.9083138
GGTAGCG3650.031.8074432
GTAGCGT4350.031.65439835
TAGCGGA3600.031.49922834
TAGCGTC4500.031.19923636
CGTCGTG4500.031.19923639
GTATACG350.004483771530.8595871
TCGTATG3350.029.01421740
AGTAGCG5000.028.619334
AGCGGAT4200.026.9993435
TACCGCT4400.026.99933829
GGTATCA45850.026.9138031
CGCTGAT3650.026.62948432
CTCGTAT3500.026.22792839
CGTATGC3950.025.97404741
Produced by FastQC (version 0.11.5)