FastQCFastQC Report
Wed 25 May 2016
SRR2074795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3411845
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3050.025.67150343
GGTATCA81000.025.5679171
ATCGCGT600.00226583922.49944937
TCGTATG3400.022.2347540
GTATCAA124550.020.2049221
ACGAGAC6900.019.95632622
CGTATGC3550.019.01361841
CCACGAG7950.018.0000920
TCTCGTA4350.017.99956138
CCCACGA7700.017.88346719
CTCGTAT3950.017.77171739
GTCTCGC5950.016.7907351
ATTGGCG3600.016.5000821
ATCTCGT4650.016.25766837
TCTGTCG5550.016.0541328
ATGCCGT4950.015.818027544
ATCAACG157850.015.8056513
TCAACGC158000.015.7222874
TATCAAC159800.015.6296752
CAACGCA162600.015.6098475
Produced by FastQC (version 0.11.5)