FastQCFastQC Report
Wed 25 May 2016
SRR2074794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115485
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2950.041.186143
TCGTATG3400.036.5291140
CGTATGC3700.033.56728741
ATTGGCG3400.033.35266521
ATCTCGT3900.028.38438237
AGCGGAT4200.027.64262635
CTCGTAT4300.027.62767839
TAGCGGA4300.027.62767834
ATTTACG3750.027.35977254
TCTCGTA4400.025.77251238
ATGCCGT4400.025.15888244
CCGTCTT4200.024.42836847
ACCCGTA901.6988961E-623.99980254
GGTAGCG5100.023.82333232
ACGAGAC5850.023.07673322
CGAGACA6050.022.31386423
CGTCTTC4850.022.26785748
TGCCGTC4900.022.04063245
CGGATGA5300.021.9054837
TATAACG1507.530616E-1021.6013532
Produced by FastQC (version 0.11.5)