FastQCFastQC Report
Wed 25 May 2016
SRR2074793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441482
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT5500.038.7808938
AGCGTCG6050.037.0404437
CGTCGTG6000.035.54915239
CGTGGTA6200.035.2733542
GTAGCGT6400.034.17105535
TAGCGTC6550.033.38851536
TATGCCG3500.032.39922743
AGTAGCG7250.030.90960734
ATTTACG2250.030.00051354
GGGTAGT8000.027.33684724
GTCGTGG8150.026.83371440
TCGTGGT8350.025.86764741
GGTATTC6150.025.46332745
ATTGGCG2900.025.13784621
TCGTATG3900.024.92248240
GGTAGTC9150.024.19614225
CTCGTAT3700.024.08050539
GTGTAAG9000.023.69943412
ATGCCGT4900.023.69379644
CGTATGC4450.023.66235441
Produced by FastQC (version 0.11.5)