FastQCFastQC Report
Wed 25 May 2016
SRR2074792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081262
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC29940.1438550264214693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA6700.043.9251442
CGTCGTG6950.043.89904439
TAGCGTC6850.042.96327236
GTAGCGT7400.040.86464335
GCGTCGT7700.039.97381638
AGTAGCG7500.039.5997934
AGCGTCG7950.038.37715537
TCGTGGT8450.035.46726641
GTCGTGG8800.034.97708540
TATGCCG2550.034.9409943
GGTATTC7300.030.32860445
CGATATT1250.030.2398411
TATCACG1700.030.1763082
GGGTAGT10050.029.55279224
TAAGCAT10550.029.4318315
GTGTAAG10550.028.91927712
GGTGTAA10900.028.23838211
GAGTAGC10900.027.74297333
GTATTCC8050.027.50295846
AAGCATC11600.027.4660316
Produced by FastQC (version 0.11.5)