FastQCFastQC Report
Wed 25 May 2016
SRR2074791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643281
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71600.030.1695211
CGTCGTG4150.027.97538439
TATGCCG3400.027.79360243
AGCGTCG4600.024.65171637
GTAGCGT4550.024.32921835
GCGTCGT4900.024.24444838
TAGCGTC4600.024.0647736
CTCGTAT4650.023.22537639
GTATCAA121000.023.1857781
ACGTTCA5650.021.98189427
AATCTCG4600.021.71698836
AGTAGCG5350.021.70053134
TCTCGTA4950.021.27233338
CGTGGTA5700.019.89436742
TCGTATG6250.019.4396440
ATGCCGT5300.018.84896744
TCGTGGT6400.018.56215541
TCAACGC153500.018.15364
ATCAACG153250.018.1479763
TATCAAC153500.018.0656472
Produced by FastQC (version 0.11.5)