FastQCFastQC Report
Wed 25 May 2016
SRR2074790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899809
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4250.036.21127740
TATGCCG4050.033.99954243
CGTATGC4050.031.9995741
CTCGTAT4950.030.54504639
TCTCGTA5100.030.17606538
ATTGGCG4050.029.3329421
ATCTCGT5700.027.47331437
TAGCGGA4600.026.41268734
TATCTCG6100.026.11440536
ATTTACG4050.026.000154
AGCGGAT4900.025.8976135
GGTATCA60750.025.3796251
ATGCCGT4950.024.5455544
TTGGCGT5100.023.8232122
TGCCGTC4950.022.9091845
GGTAGCG5550.022.8645632
ACGAGAC7950.022.75441422
CCCACGA8650.022.47368819
CGTATGA1355.2623363E-921.99970641
GTATCAA96800.021.14411
Produced by FastQC (version 0.11.5)