FastQCFastQC Report
Wed 25 May 2016
SRR2074787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102946
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC55030.2616805186628663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.11450603106308958No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.11365008897042529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG10100.047.04925539
AGCGTCG10550.045.8101837
GTAGCGT10550.044.7864935
CGTGGTA10700.044.15864642
TAGCGTC10650.044.11244636
GCGTCGT10850.044.0458538
AGTAGCG11350.042.58127234
TCGTGGT11600.040.2670341
GGTATTC9900.039.81797445
GTCGTGG12550.038.0794840
GGGTAGT13400.036.46995524
GTATTCC10750.035.9160946
GTGTAAG13750.035.3452712
CTGGGTA14050.034.97490722
TAAGCAT14000.034.71410415
GTAAGCA14150.034.34610714
CTCGTAT2850.034.10508339
GGTAGTC14350.033.67926425
TGGTGTA14500.033.51706310
GGTGTAA14750.032.94897511
Produced by FastQC (version 0.11.5)