FastQCFastQC Report
Wed 25 May 2016
SRR2074786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137849
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC40550.18967663291467263No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACG26020.12171112178643113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT8000.044.88664235
TAGCGTC8150.044.39179236
GCGTCGT8150.044.39179238
AGCGTCG8450.043.77431537
CGTCGTG8350.043.32851839
AGCGGAT5300.042.28221535
TAGCGGA5300.041.7727934
CGTGGTA8600.041.12712542
ATTGGCG5100.040.23452821
ATTTACG4950.039.8174254
AGTAGCG9200.039.6188134
GGTAGCG5850.036.9223732
GGTATTC7950.036.67854345
GGGTAGT10100.035.01913524
GTCGTGG10650.033.97118440
TCGTGGT10450.033.84624541
GTAGCGG6800.031.764133
GTGTAAG11500.031.22841312
TGGGTAG11700.030.92248723
TGGTGTA11550.030.62422410