FastQCFastQC Report
Wed 25 May 2016
SRR2074785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100557
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC71590.2308939974333644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA16100.044.77532642
GTAGCGT16100.044.60762835
TAGCGTC16000.044.54893536
AGCGTCG16200.044.4989437
CGTCGTG16250.044.19586639
GCGTCGT16450.043.6585338
AGTAGCG17800.040.65071534
TCGTGGT17950.039.8597741
GTCGTGG18900.038.1419540
GGGTAGT19600.036.91808324
GGTATTC15300.036.000345
TATGCCG5300.035.65952743
GTGTAAG20950.035.05405812
GTAGTCT21150.033.95663526
GTATTCC16050.033.6451446
CTGGGTA21150.033.57420722
TAAGCAT22050.033.306415
GAGTAGC21900.033.16359333
CTGAGTA22150.032.78928431
GTAAGCA22550.032.6871114
Produced by FastQC (version 0.11.5)