FastQCFastQC Report
Wed 25 May 2016
SRR2074784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172759
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC40580.12790129978356377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG8500.039.70481539
CGTGGTA8750.038.57039642
GTAGCGT9000.037.49899335
GCGTCGT9250.036.77739338
TAGCGTC9450.035.4276236
AGTAGCG9800.034.98885734
AGCGTCG9750.034.89137337
TCGTGGT10500.032.3991341
GGTATTC9700.028.94767645
GTCGTGG12050.028.67972840
GGGTAGT11850.028.25240724
GGTATCA56900.026.2891751
GTATTCC11850.024.1512546
GTGTAAG14350.024.08449612
TCTCGTA5050.024.0587638
GGTGTAA15050.023.3234711
TATGCCG5150.023.06734343
TGAGTAG14900.023.01280432
GGTAGTC14700.022.95856725
CTCGTAT5300.022.92391239
Produced by FastQC (version 0.11.5)