FastQCFastQC Report
Wed 25 May 2016
SRR2074783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328871
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG6750.028.7996821
TAGCGGA6800.028.58791734
AGCGGAT7250.027.55831535
ATTTACG6200.027.43517954
GGTAGCG7850.026.13983732
GGTATCA69350.023.6334571
GCGTGAA8400.021.856925
TTGGCGT8800.021.47703422
GTAGCGG9550.020.92123433
TATGCCG2850.020.84187343
CGGATGA10250.020.54611437
GCGGATG10300.020.44637536
GTATCAA106750.019.906361
GGCGCCA9500.019.89481415
TGAGGCG10250.019.75617612
AGGTAGC10800.019.24978631
GAGGCGC10600.018.84912913
AAGGTAG10950.018.49294530
GCGCCAT10650.017.74654816
CGCCATT10550.017.14698617
Produced by FastQC (version 0.11.5)