FastQCFastQC Report
Wed 25 May 2016
SRR2074782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833029
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC72200.3938835664902192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA13750.048.10824242
CGTCGTG14050.047.6575239
GTAGCGT13900.047.5890935
TAGCGTC14150.046.7482936
AGTAGCG14400.046.49917634
AGCGTCG14650.045.88997737
GGTATTC11250.045.59919445
GTATTCC11350.044.9595646
GTCGTGG14950.044.78850640
GCGTCGT14950.044.78850638
TCGTGGT14850.044.54466641
TAAGCAT15950.042.6598815
GTAAGCA17150.040.30359314
GGGTAGT17050.040.22326324
GTGTAAG17400.040.03486612
AAGCATC17100.039.7909416
CTGGGTA16950.039.50588222
TGGTATT16150.039.45441444
GGTGTAA18000.038.5503711
TGGTGTA17800.038.5284610
Produced by FastQC (version 0.11.5)