FastQCFastQC Report
Wed 25 May 2016
SRR2074781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138127
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC37410.1749662204349882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT8200.043.79194335
TAGCGTC8300.043.5896336
GCGTCGT8400.043.070738
CGTGGTA8700.041.27516642
AGCGTCG9250.040.280437
ATTGGCG4600.039.3263421
TAGCGGA4750.038.65197834
ATTTACG4350.038.4821154
CGTCGTG9600.037.9681139
AGTAGCG9550.037.8841834
GGGTAGT10550.036.5965424
GGTAGCG5300.036.169232
AGCGGAT5250.035.48511535
GGTATTC8250.034.69032345
TCGTGGT10850.034.3404341
GTAGCGG5550.034.0534833
TATGCCG3100.033.0962243
TGGGTAG11600.032.8184123
GTGTAAG11650.032.2163112
GTCGTGG12100.032.1316940
Produced by FastQC (version 0.11.5)