FastQCFastQC Report
Wed 25 May 2016
SRR2074780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270694
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC74150.32655214661244536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT14800.046.8845835
CGTCGTG14550.046.57676739
TAGCGTC14750.046.49436636
CGTGGTA14850.045.63582642
AGCGTCG15100.045.59548637
AGTAGCG15750.044.91375434
GCGTCGT15150.044.91035538
GGTATTC13150.042.50139645
TCGTGGT16300.041.0792741
GTCGTGG16950.039.98182740
TAAGCAT17300.039.64114815
GTGTAAG17600.039.58082212
GGGTAGT17000.039.54659324
GTATTCC14150.038.54371346
GGTGTAA18550.037.69932611
CTGGGTA18150.036.89212422
GTAAGCA18900.036.8567114
TGGGTAG18250.036.6899823
GAGTAGC19300.036.65241633
GTTTCAT19200.035.71992
Produced by FastQC (version 0.11.5)