FastQCFastQC Report
Wed 25 May 2016
SRR2074777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882506
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG2800.042.42763529
CTCGACG3150.039.42770428
ATCGCGT359.6532356E-538.57276537
CGACGGC3050.037.1795130
GACGGCA3100.036.57983831
AGACTCG3300.035.1810425
ACGGCAT2950.034.77889332
ACTCGAC3200.034.59298727
TAGCGGA1500.034.19924534
ATTGGCG1450.033.51650221
ATTTACG1300.033.23003854
GACTCGA3450.032.86884326
GGTAGCG1600.032.06179432
GCGTCGT1450.031.6562738
CGGCATC3200.031.21806133
CTCGTAT1300.031.15492439
CGTCGTG1500.030.60106339
TAACGCA1250.030.242764
CGTATGA454.2246876E-430.00103841
CATTCCG454.22609E-429.999349
Produced by FastQC (version 0.11.5)