FastQCFastQC Report
Wed 25 May 2016
SRR2074776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260393
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACG24590.10878639245476339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA5700.043.1040134
ATTGGCG5650.040.62008321
TATGCCG4350.039.7229843
TCGTATG4700.037.91378840
CGTATGC4850.036.741241
AGCGGAT6650.036.5402935
ATTTACG6300.035.5711854
GGTAGCG7400.033.56659332
GTAGCGG7600.033.3937733
GTACCGT502.0634077E-532.4019246
CTCGTAT5550.032.10717439
ATGCGAC1850.030.64911514
ATCTCGT5550.030.64775737
TCTCGTA5750.030.52085138
TGCGACA2050.030.29314415
TTGGCGT7450.030.08032622
GAGGCGC8400.029.57187513
ATGCCGT5650.029.15023844
AAGGTAG8650.029.02805730
CCGTCTT5500.028.96343447
Produced by FastQC (version 0.11.5)