FastQCFastQC Report
Wed 25 May 2016
SRR2074775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309893
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC38540.16684755527636994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT8950.041.02725635
TAGCGTC9050.040.2755836
CGTCGTG9100.039.75758739
AGTAGCG9500.039.2204134
CGTGGTA8850.038.7451342
AGCGTCG9750.038.49167337
GCGTCGT9800.036.64225438
TCGTGGT10200.034.675941
GGTATTC8400.034.39303645
GGGTAGT11300.032.01717424
GGTAGTC11850.031.21467425
TATGCCG3500.030.85663443
GTGTAAG11900.030.17662812
GTCGTGG12250.029.75461240
GTATTCC9600.029.53140446
TGGTGTA12000.029.25015310
TGGGTAG12700.029.12550523
TCTCGTA3900.029.07644538
TAAGCAT12450.028.62665715
GAGTAGC13250.028.1202933
Produced by FastQC (version 0.11.5)