FastQCFastQC Report
Wed 25 May 2016
SRR2074774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227173
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC47820.2147116546402098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT10900.042.3565335
CGTCGTG11050.041.53721639
CGTGGTA10800.041.24876442
AGTAGCG11450.040.7935434
TAGCGTC11450.040.32193836
AGCGTCG11950.039.08669737
GCGTCGT11800.038.8971438
TCGTGGT12000.037.5738841
GGTATTC10000.036.71972345
GTCGTGG13350.034.78547740
GTAAGCA14850.033.27322814
GGGTAGT14450.032.51186824
GAGTAGC15100.032.1844733
TAAGCAT15200.031.97416115
GTATTCC11500.031.93019546
CTGGGTA15450.030.93249922
GTGTAAG15050.030.85760312
CTGAGTA15700.030.61054831
GGTGTAA15500.030.4843311
TGGTATT14150.030.14818444
Produced by FastQC (version 0.11.5)