FastQCFastQC Report
Wed 25 May 2016
SRR2074773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412422
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC48540.2012085779353695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA10500.042.6850642
CGTCGTG11350.041.867239
GCGTCGT11450.041.5015538
GTAGCGT11400.040.2625435
TAGCGGA5000.039.41939534
ATTTACG4600.039.3254854
AGCGTCG11950.039.313237
TAGCGTC11700.039.23016736
ATTGGCG4950.038.18202221
GGTAGCG5300.037.69753332
AGTAGCG12450.037.30063234
TATGCCG4750.036.37838743
TCGTGGT12750.035.9994541
GTCGTGG13950.034.25753840
GTAAGCA14100.034.0852914
GGGTAGT14050.034.01371424
CTGGGTA14300.033.98549322
AGCGGAT5650.033.9286835
GTAGCGG6000.033.2994933
CTCGTAT5400.032.99949339
Produced by FastQC (version 0.11.5)