FastQCFastQC Report
Wed 25 May 2016
SRR2074772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866023
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC6200.038.75742736
CGTCGTG6200.037.01552239
GTAGCGT6600.036.81757435
AGCGTCG7100.034.2247937
AGTAGCG7350.033.42802434
CGTGGTA6750.032.79945842
GCGTCGT7250.031.6546538
GGTATCA55800.029.5171871
TCGTGGT8500.026.99955641
GTCGTGG8400.026.99955640
GGTATTC7500.025.56002445
GGGTAGT10050.024.44778624
TATGCCG4000.024.299643
GTATCAA92700.022.0493341
GTATTCC8900.020.93260446
GGTAGTC11500.020.89530825
TGTAAGC11000.020.86365513
ACTACCG4950.020.7269327
GTAAGCA11350.020.69569414
GGTGTAA11650.020.62662911
Produced by FastQC (version 0.11.5)