FastQCFastQC Report
Wed 25 May 2016
SRR2074771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993456
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC25220.12651395365636361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4800.043.31193539
GCGTCGT5250.040.1137638
GTAGCGT5100.039.70536835
CGTGGTA5500.038.2904142
AGCGTCG5300.038.2070537
TAGCGTC5450.037.15548336
ATTTACG2950.034.7792154
ATTGGCG3000.033.29956421
TAGCGGA3100.033.09634434
AGTAGCG6450.032.65073834
TCGTGGT6550.032.15225241
GTCGTGG6500.031.984240
AGCGGAT3350.031.43242635
TATGCCG2750.031.41777243
GGGTAGT6850.030.74412524
GGTATTC5250.028.79962345
GTGTAAG7750.026.82613212
GGTAGCG4150.026.02375832
TAACGCA1158.221832E-1025.8270474
TCGTATG2950.025.62678540
Produced by FastQC (version 0.11.5)