FastQCFastQC Report
Wed 25 May 2016
SRR2074769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909508
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3900.025.61479240
GGTATCA82250.024.851181
TATGCCG4150.024.72231943
CGTATGC4750.021.0310941
TCTCGTA4800.020.2495338
CTCGTAT4700.020.10591539
GTATCAA128650.020.0648141
ATGCCGT5550.019.45950744
ACGAGAC8800.019.32959222
ATCTCGT5400.018.4995737
CCCACGA9700.017.25777219
CCACGAG9900.016.90913220
ATCAACG156050.016.161073
TCAACGC155350.016.1469864
CAACGCA160200.016.0797185
TATCAAC157150.016.0479472
AACGCAG167500.015.4917756
CACGAGA10650.015.46482621
TGCCGTC5900.015.10173145
GTACATG155400.014.8733821
Produced by FastQC (version 0.11.5)